| Name | Time | Date | Meet |
|---|---|---|---|
| Sebastian Wolff | 43.64 | Feb. 25, 2023 | Ivy League Championships (M) |
| Jack Mahoney | 43.83 | March 2, 2019 | Ivy League Championships (M) |
| JACK Brenneman | 44.19 | Feb. 27, 2016 | 2016 Ivy League Championships (M) |
| Wes Newman | 44.25 | March 8, 2008 | 2008 EISL Championship |
| Wes Newman | 44.51 | Feb. 24, 2007 | 2007 EISL Championships |
| Mike Smit | 45.08 | March 3, 2005 | 2005 EISL Championships |
| Jeremy Sample | 45.90 | March 4, 2004 | 2004 Men's EISL Championships |
| Charlie Ernst | 46.41 | Feb. 28, 2002 | 2002 Men's EISL Championships |
