| Time | Date | Name | Meet |
|---|---|---|---|
| 43.45 | Feb. 22, 2020 | Gustavo Santos | American Athletic Conference Championships |
| 43.71 | Feb. 20, 2016 | Michael Dugan | American Conference Championships |
| 43.75 | Feb. 21, 2015 | Nikola Simic | American Conference Championships |
| 44.13 | March 1, 2014 | Nikola Simic | Conference USA Championships |
| 44.59 | Feb. 18, 2012 | Nikola Simic | Conference USA Championships |
| 44.62 | Nov. 19, 2011 | Nikola Simic | North Carolina Invitational |
| 45.53 | Nov. 2, 2011 | Nikola Simic | East Carolina vs. North Carolina State |
| 45.81 | Feb. 26, 2011 | Geoffrey Bobsin | Conference USA Championships |
| 46.14 | Feb. 27, 2010 | Geoffrey Bobsin | Conference USA Championships |
| 46.20 | Feb. 28, 2009 | Junior Temotio | Conference USA Championships - Day Four - Finals |
| 46.38 | Oct. 11, 2008 | Junior Temotio | Xavier, East Carolina, West Virginia @Marshall |
