| Name | Time | Date | Meet |
|---|---|---|---|
| Sydney Ulmer | 26.60 | Feb. 17, 2024 | Michigan First Chance Meet |
| Delaney Duncan | 27.24 | March 22, 2019 | NCAA Division I Womens Championships |
| Delaney Duncan | 27.63 | March 1, 2019 | Mid-American Championships (W) |
| Delaney Duncan | 28.73 | Oct. 20, 2018 | Eastern Michigan vs. Denison |
| Emily Wimmer | 30.24 | Feb. 13, 2016 | Ohio State Invitational |
| Genna Worthley | 31.10 | Jan. 4, 2014 | Florida International Invitational |
| Jessica Cavaiani | 31.32 | Jan. 5, 2013 | FIU Invitational |
