| Name | Time | Date | Meet |
|---|---|---|---|
| Carly Jackson | 54.19 | Feb. 26, 2016 | 2016 Mid-American Championships (W) |
| Carly Jackson | 54.47 | Feb. 27, 2015 | 2015 Mid-American Championships (W) |
| Jackie Swartz | 54.58 | Dec. 1, 2012 | Eastern Michigan Invitational |
| Meghan Richardson | 55.67 | Feb. 24, 2012 | Mid-American Conference Womens Championships |
| Elisabeth Torres | 55.72 | Feb. 21, 2009 | 2009 Mid-American Conference Championships - Day Three - Prelims |
| Samantha Nameth | 57.12 | Nov. 1, 2008 | Eastern Michigan @ Toledo |
