| Time | Date | Name | Meet |
|---|---|---|---|
| 49.86 | Feb. 8, 2023 | Julian Najarro Sedar | Great Midwest-Mountain East Conference Championship |
| 50.54 | April 1, 2021 | Andrew Creelman | GMAC & MEC Championship |
| 52.96 | Feb. 12, 2020 | Anthony Giuseppetti | Great Midwest-Mountain East Conference Championship |
| 53.05 | March 5, 2017 | Christian March | Eastern College Athletic Conference Championships |
| 54.10 | Feb. 27, 2016 | Christian March | ECAC Time Trials |
| 54.61 | Feb. 16, 2013 | Caleb Stair | Capital Athletic Conference Inc. Championships |
| 56.11 | Feb. 4, 2012 | Caleb Stair | Marymount (Virginia) vs. Frostburg State |
| 56.30 | Nov. 19, 2011 | Caleb Stair | Randolph Macon Invitational |
| 56.74 | Feb. 20, 2010 | Caleb Stair | Grove City Invitational |
| 57.14 | Dec. 6, 2009 | Caleb Stair | Blue Devil Invitational |
| 59.14 | Oct. 23, 2009 | Caleb Stair | Clarion Fall Classic |
| 1:02.58 | Oct. 25, 2008 | Jack Hudson | Clarion Fall Classic |
