| Name | Time | Date | Meet |
|---|---|---|---|
| Daniela Johnson | 51.07 | Nov. 10, 2011 | 2011-12 Florida High School Athletic Association Class 3A Championships |
| Hannah Kling | 52.71 | Nov. 13, 2008 | 2008-09 Florida Class 3A State Championships |
| Brittany Selts | 54.03 | Nov. 3, 2006 | FHSAA - Class 3A Finals- USA- |
| Monica Singletary | 54.52 | Nov. 21, 2002 | FHSAA 3A Championships |
