| Time | Date | Name | Meet |
|---|---|---|---|
| 49.24 | March 17, 2022 | Mitchell Mages | NCAA Division III Championship |
| 49.66 | Feb. 21, 2020 | Nick Seroni | College Conference of Illinois & Wisconsin Championships |
| 49.83 | Feb. 15, 2019 | Nick Seroni | College Conference of Illinois & Wisconsin Championships |
| 50.05 | Feb. 19, 2010 | Doug Schranck | College Conference of Illinois & Wisconsin Championships |
| 50.31 | Feb. 15, 2008 | Bryce Davis | CCIW Championships |
| 50.99 | Feb. 9, 2007 | Bryce Davis | CCIW Championships |
| 51.26 | Feb. 10, 2006 | Bryce Davis | CCIW Swimming Championships |
| 51.28 | Feb. 11, 2005 | Henry Somers | CCIW Championships |
| 53.68 | Feb. 14, 2003 | Casey Griffiths | CCIW Swimming Championships |
