| Time | Date | Name | Meet |
|---|---|---|---|
| 49.33 | Feb. 24, 2024 | Emma Feltzer | Missouri Valley Conference Championships |
| 49.88 | Feb. 18, 2023 | Emma Feltzer | Missouri Valley Conference Championships |
| 49.93 | Feb. 21, 2015 | Marissa Yontz | Missouri Valley Conference Championships |
| 50.16 | Dec. 7, 2014 | Marissa Yontz | Zippy Invitational |
| 50.44 | Feb. 15, 2014 | Marissa Yontz | Missouri Valley Conference Championships |
| 50.63 | Dec. 8, 2013 | Marissa Yontz | Zippy Invitational |
| 51.22 | Feb. 16, 2013 | Marissa Yontz | Missouri Valley Conference Championships |
| 51.66 | Feb. 18, 2012 | Katherine Nelson | Missouri Valley Conference Championships |
| 51.94 | Feb. 13, 2010 | Jeanette Nolte | Missouri Valley Conference Championships |
| 53.48 | Feb. 14, 2009 | Lauren Boyd | Missouri Valley Conference Championships - Day Three - Finals |
