| Time | Date | Name | Meet |
|---|---|---|---|
| 24.29 | March 5, 2021 | Will Myhre | B1G Men's Conference Championships |
| 24.52 | March 25, 2016 | Roman Trussov | NCAA Division I Mens Championships |
| 24.73 | Feb. 5, 2016 | Roman Trussov | Iowa vs. Western Illinois |
| 25.53 | Feb. 6, 2015 | Roman Trussov | Iowa vs. Northern Iowa, Western Illinois |
| 25.56 | Feb. 7, 2014 | Roman Trussov | Iowa vs. Western Illinois |
| 26.01 | Feb. 8, 2013 | Michael Nelson | Iowa vs. Western Illinois, Northern Iowa |
| 26.70 | Feb. 3, 2012 | Sean Swibes | Iowa vs. Western Illinois |
| 26.76 | Feb. 4, 2011 | Andrew Marciniak | Iowa vs. Northern Iowa, Western Illinois |
| 26.86 | Feb. 5, 2010 | Matthew Ryan | Iowa vs. Western Illinois, Northern Iowa |
