| Time | Date | Name | Meet |
|---|---|---|---|
| 46.92 | March 16, 2023 | Liam McDonnell | NCAA Division III Championship |
| 47.12 | Nov. 18, 2022 | Liam McDonnell | Kenyon Total Performance Invitational |
| 47.13 | March 17, 2022 | Liam McDonnell | NCAA Division III Championship |
| 47.21 | Feb. 18, 2022 | Liam McDonnell | Ohio Athletic Conference Championships |
| 48.15 | March 26, 2021 | Liam McDonnell | Ohio Athletic Conference Championships |
| 48.49 | Feb. 14, 2020 | Forrest Campbell | Ohio Athletic Conference Championships |
| 48.87 | Feb. 13, 2020 | Forrest Campbell | Ohio Athletic Conference Championships |
| 48.92 | Feb. 15, 2019 | Jackson Cooper | Ohio Athletic Conference Championships |
| 50.09 | March 20, 2014 | David Holvey | NCAA Division III Championships |
| 50.57 | Feb. 14, 2014 | David Holvey | Ohio Athletic Conference Championships |
| 51.54 | Feb. 15, 2013 | David Holvey | Ohio Athletic Conference Championships |
| 51.86 | Feb. 17, 2012 | David Holvey | Ohio Athletic Conference Championships |
| 52.15 | Feb. 19, 2010 | Eric Davis | Ohio Athletic Conference Championships |
| 54.19 | Nov. 7, 2009 | Eric Davis | John Carroll vs. Mount Union |
| 55.50 | Oct. 25, 2008 | Eric Davis | Ohio Northern @John Carroll |
