| Time | Date | Name | Meet |
|---|---|---|---|
| 50.49 | March 25, 2022 | Caspar Corbeau | NCAA Division I Mens Championship |
| 50.68 | March 24, 2017 | Will Licon | NCAA Division I Mens Championships |
| 51.15 | Feb. 22, 2017 | Will Licon | Big XII Conference Time Trials |
| 52.22 | March 27, 2010 | Scott Spann | NCAA Division I Mens Championships |
| 52.61 | March 15, 2007 | Tyler Ohalloran | NCAA Division I Mens Championships |
| 54.81 | March 6, 2003 | Christian Schurr | American Short Course Championships |
