| Name | Time | Date | Meet |
|---|---|---|---|
| Madeleine Hebert | 50.03 | April 15, 2023 | Mt. Sac Meet of Champions |
| Ana Schulz | 52.00 | April 14, 2023 | Mt. Sac Meet of Champions |
| Alex Rodman | 53.68 | May 14, 2013 | 2013 SI CIF - San Diego Sectional |
| Ashley Young | 53.71 | May 18, 2010 | 2010 CIF San Diego Section - Division II Championships |
| Ashley Young | 53.12 | May 19, 2009 | 2009 CIF San Diego Division II Championships |
| Molly Patrick | 54.05 | May 16, 2006 | CIF - San Diego Section - Division II |
