| Name | Time | Date | Meet |
|---|---|---|---|
| Malaika Schneider | 28.84 | Feb. 17, 2024 | Sunshine State Conference Championships |
| Malaika Schneider | 29.05 | Nov. 17, 2023 | House of Champions Invitational |
| Ewa Osiniak | 29.40 | Feb. 15, 2020 | Sunshine State Conference Championships |
| Sarah Hughes | 29.64 | Feb. 23, 2019 | Sunshine State Conference Championships |
| Rebecca Matthews | 30.39 | Jan. 3, 2015 | FIU Invitational |
| Tyne Potgieter | 31.40 | Jan. 17, 2014 | St. Leo vs. Florida Tech, Lynn |
