| Name | Time | Date | Meet |
|---|---|---|---|
| Luna Mertins | 24.30 | March 10, 2022 | NCAA Division II Championship |
| Luna Mertins | 24.94 | Feb. 18, 2022 | Sunshine State Conference Championships |
| Clara Monges | 26.06 | Feb. 22, 2019 | Sunshine State Conference Championships |
| Clara Monges | 26.47 | Nov. 30, 2018 | SCAD Invitational |
| Rebecca Matthews | 26.63 | Jan. 9, 2016 | Lynn Sprint Invitational |
| Thalie Carmigniani | 26.84 | Jan. 3, 2015 | FIU Invitational |
| Magdalena Baranowska | 27.30 | Jan. 17, 2014 | St. Leo vs. Florida Tech, Lynn |
