| Name | Time | Date | Meet |
|---|---|---|---|
| Maurice Grabowski | 43.53 | March 16, 2024 | NCAA Division II Championship |
| Maurice Grabowski | 44.51 | Feb. 18, 2024 | Sunshine State Conference Championships |
| Maxim Tsyfarov | 44.58 | Nov. 18, 2023 | House of Champions Invitational |
| Julian Chan Quee Lin | 44.88 | Feb. 20, 2022 | Sunshine State Conference Championships |
| Dalton Brown | 46.18 | Dec. 7, 2019 | SCAD Invitational |
| Aleksander Olejnik | 48.36 | Oct. 12, 2019 | NSU Shark Invitational |
