| Name | Team | Time | Score | FINA | Imp | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Olivia Smoliga |
 Athens Bulldogs
|
25.19 | 20.00 | 836 | +0.2% | SB | |
| 5 | Madison Kennedy |
 SwimMAC Carolina
|
25.22 | 14.00 | 833 | -0.9% | ||
| 2 | Courtney Caldwell |
 Wolfpack Elite
|
25.24 | 17.00 | 831 | +1.8% | PB | |
| 3 | Caitlin Cooper |
 Cavalier Swimmin
|
25.57 | 16.00 | 799 | +1.0% | SB | |
| 4 | Veronica Burchill |
Athens Bulldogs
|
25.86 | 15.00 | 773 | -0.6% | ||
| 6 | Andi Murez |
 Israel
|
25.89 | 13.00 | 770 | 0.0% | ||
| 7 | Alyssa Marsh |
 Duke Aquatics
|
26.10 | 12.00 | 752 | 0.0% | ||
| 9 | Julie Woody |
 Savannah Swim Team
|
26.32 | 9.00 | 733 | +0.7% | PB | |
| 10 | Zhada Fields |
 North Carolina Aquatic Club
|
26.46 | 7.00 | 721 | +0.6% | PB | |
| 11 | Ky-lee Perry |
Wolfpack Elite
|
26.47 | 6.00 | 720 | -4.3% | ||
| 8 | Kathryn Ford |
North Carolina Aquatic Club
|
26.51 | 10.00 | 717 | -1.0% | ||
| 12 | Lauren Rhodes |
Queens Universit
|
26.58 | 5.00 | 712 | +0.4% | SB | |
| 13 | Maddie Hess |
Duke Aquatics
|
26.67 | 4.00 | 704 | +0.3% | SB | |
| 8 | Melanie Margalis |
 Saint Petersburg Aquatics
|
26.74 | 10.00 | 699 | -0.6% | ||
| 14 | Jordan Stout |
Athens Bulldogs
|
26.79 | 3.00 | 695 | +0.1% | SB | |
| 15 | Maddy Flickinger |
SwimMAC Carolina
|
26.99 | 2.00 | 680 | -0.1% | ||