| Name | Team | Time | FINA | |||
|---|---|---|---|---|---|---|
| 1 | Lillian Grebner |
 Aberdeen Swim Club
|
1:04.42 | 528 | ||
| 2 | Madeline Kenyon |
 Dayton Raiders
|
1:04.47 | 527 | ||
| 3 | Kayla Effinger |
Dayton Raiders
|
1:04.54 | 525 | ||
| 4 | Hunter Waterman |
 Team Vortex
|
1:04.59 | 524 | ||
| 5 | Leslie Gilsdorf |
Team Vortex
|
1:04.90 | 516 | PB | |
| 6 | Anna McCarty |
Dayton Raiders
|
1:04.99 | 514 | PB | |
| 7 | Halle Kuck |
Aberdeen Swim Club
|
1:05.93 | 493 | ||
| 8 | Elena Leiva |
 Mid Wisconsin Wave Makers
|
1:05.95 | 492 | ||
| 9 | Anna Schmidt |
Aberdeen Swim Club
|
1:06.16 | 488 | ||
| 10 | Demetra Fellows |
Vipers of Albuquerque Swim Team
|
1:08.34 | 442 | ||
| 11 | Taylor Studer |
 Dakota Riptide
|
1:10.09 | 410 | ||
| – | Ariana Saghafi |
 Wisconsin Aquatics
|
NS | – | ||