| Name | Team | Time | FINA | Imp | |||
|---|---|---|---|---|---|---|---|
| 1 | Kasey Milstroh |
 St. Cloud Area Family YMCA
|
27.00 | 679 | -0.3% | ||
| 2 | Lillian Kurzydlo |
Coho Swim Club
|
28.73 | 563 | -0.9% | ||
| 3 | Kady Harris |
Sioux Falls Swim
|
28.92 | 552 | – | ||
| 3 | Mindy Wilkes |
Medina Recreation Swim Team
|
28.92 | 552 | – | ||
| 5 | Taylor Valade |
 Team Vortex
|
29.36 | 528 | – | ||
| 6 | Taylor Beagle |
Black Hills Gold
|
29.41 | 525 | – | ||
| 7 | Emma Clark |
Black Hawk
|
29.48 | 522 | – | ||
| 8 | Olivia Muchewicz |
Medina Recreation Swim Team
|
29.52 | 519 | – | ||
| 9 | Sydney Tunell |
 Rio Salado Swim Club
|
29.66 | 512 | – | ||
| 10 | Maggie Bice |
Des Moines Swimming Federation
|
29.90 | 500 | – | ||
| 11 | Kayla Conn |
 Mid Wisconsin Wave Makers
|
30.20 | 485 | – | ||
| 12 | Brigette Eagar |
Piranha Swimming
|
30.26 | 482 | – | PB | |
| 13 | Taylor Purvis |
Sea
|
30.27 | 482 | – | ||
| 14 | Elizabeth Myers |
Sea
|
30.42 | 475 | – | ||
| 15 | Carson Slater |
Des Moines Swimming Federation
|
30.85 | 455 | – | ||
| 16 | Addison Bertram |
 Dakota Riptide
|
30.91 | 452 | – | ||
| 17 | Lindsey Blommers |
Sea
|
30.93 | 452 | – | ||