| Rank | Name | Team | Time | Points | Improvement | |
|---|---|---|---|---|---|---|
| 1 | Kelsi Dahlia |
|
51.51 | 915 | – | |
| 2 | Marne Erasmus |
|
52.60 | 859 | – | |
| 7 | Mallory Comerford |
|
53.83 | 801 | – | PB |
| 3 | Kaitlin Grover |
|
53.90 | 798 | – | |
| 8 | Sharlene Brady |
|
54.35 | 779 | – | PB |
| 4 | Anna Patterson |
|
55.02 | 750 | +5.1% | SB |
| 9 | Kathryn Kinnear |
|
55.16 | 745 | – | |
| 10 | Gracie Redding |
|
55.32 | 738 | +2.4% | PB |
| 5 | Megan Brown |
|
55.40 | 735 | – | PB |
| 11 | Madeline Hoch |
|
55.79 | 720 | – | PB |
| 6 | Micaela Roemer |
|
57.18 | 669 | +1.3% | SB |
| 12 | Catherine Maxey |
|
58.08 | 638 | +0.2% | SB |